将自主研发的新型光敏剂叶绿酸f与T24细胞共同孵育后,以650 nm激光照射，通过MTT法检测光动力处理后T24细胞的生长抑制率，流式细胞术检测凋亡情况，同时利用激光共聚焦显微镜488 nm和405 nm双波长激发，应用线粒体探针,进行叶绿酸f的亚细胞定位。结果显示叶绿酸f为浓度2.5，5和10 μg/mL对T24细胞生长抑制率在光能量密度4 J/cm2下为17.68%，49.35%和84.42%，在1 J/cm2时为4.34%，37.42%和78.38%；流式细胞术显示T24细胞光动力处理后，凋亡率为(45.23±1.2)%，显著高于对照组；激光共聚焦显微镜显示504 nm激发叶绿酸f发出的红光与488 nm激发线粒体探针产生的绿光分布基本一致。实验证明叶绿酸f对T24细胞杀伤效果明显，主要是通过作用于线粒体，诱导细胞凋亡来完成的。

Water-soluble CdTe quantum dots (QD600) were synthesized by hydrothermal route. The uptake of QD600 in living cells of euglena gracilis was studied. The luminescence spectra show that the QD600 were bound in cells after incubation. The fluorescence images, measured by confocal laser scanning microscope, demonstrate that the QD600 can penetrate into the cells. The amount of cell-bound QD600 increased with incubation time of QD600 at 25 Celsius deg., but no detectable QD600 were found in the cells when the incubation temperature was 4 Celsius deg., it was suggested that the QD600 were actively taken into the cells by endocytotic pathway. These results indicate that the water-soluble CdTe quantum dots have the potential in the application of bio-labeling.

Hydrophilic photo luminescent semiconductor quantum dots (QDs) are novel nanometer-size probes, which may have potential using in bio-imaging for biological objects. In this work, the photo-stability of these QDs in two kinds of living cells was studied, compared with conventional biological probes such as fluorescein isothiocyanate (FITC) and green fluorescence protein (GFP). It was found that the concentration of QDs in living cells is the dominant factor for its photo-stability in biological environment. When the concentration of the intracellular QDs was high, the QDs show good photo-stability that is much better than the organic fluorescent probes. However when its concentration was low, the QDs also can be photo-bleached quickly. Thus the reaching of the certain concentration level is the critical condition for QDs in the application of bio-imaging.

The effects of laser irradiation on erythrocyte deformability and related mechanism were investigated. Membrane attached hemoglobin (Hbm) is the determinant factor of erythrocyte deformability. After irradiation of 532 nm (30 mW, 20 minutes) or 632.8 nm (30 mW, 60 minutes), the content of Hbm lowered and the deformability of erythrocytes increased, indicating that the decreasing of Hbm is one of the mechanisms of laser action to improve the erythrocyte deformability. The 532-nm laser is more efficient than 632.8 nm laser in the action, which is consistent with the absorption spectrum of Hbm, confirming that the Hbm is the target molecule under laser irradiation.